Composite

Part:BBa_K4129132

Designed by: Magnus Haahr   Group: iGEM22_DTU-Denmark   (2022-10-10)


The fungal synthetic expression system with FunsTF65 and 6xLexO-Pmin


Usage and Biology

This composite part was used to characterise the synthetic transcription factor (sTF) FunsTF65. The reporter cassette consists of the synthetic 6xLexO-Pmin (BBa_K4129120) that transcribe mCherry.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4186
    Illegal XbaI site found at 6099
    Illegal PstI site found at 141
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 911
    Illegal NheI site found at 2986
    Illegal PstI site found at 141
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 145
    Illegal BglII site found at 3173
    Illegal BamHI site found at 2920
    Illegal BamHI site found at 5380
    Illegal XhoI site found at 701
    Illegal XhoI site found at 1083
    Illegal XhoI site found at 4050
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 4186
    Illegal XbaI site found at 6099
    Illegal PstI site found at 141
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4186
    Illegal XbaI site found at 6099
    Illegal PstI site found at 141
    Illegal NgoMIV site found at 3078
    Illegal NgoMIV site found at 6016
    Illegal AgeI site found at 1290
    Illegal AgeI site found at 1438
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1117
    Illegal BsaI.rc site found at 5730
    Illegal SapI site found at 323


[edit]
Categories
Parameters
None